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POLR3D Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55887

Novus Biologicals, part of Bio-Techne
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NBP2-55887
NBP2-55887-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PRPLLTGARGLIGRRPAPPLTPGRLPSIRSRDLTLGGVKKKTFTPNIISRKIKEEPK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit POLR3D Antibody - BSA Free (NBP2-55887) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for POLR3D Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: POLR3D Antibody [NBP2-55887]

Immunocytochemistry/ Immunofluorescence: POLR3D Antibody [NBP2-55887]

Immunocytochemistry/Immunofluorescence: POLR3D Antibody [NBP2-55887] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm.
POLR3D Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: POLR3D Antibody - BSA Free [NBP2-55887]

Chromatin Immunoprecipitation-exo-Seq: POLR3D Antibody - BSA Free [NBP2-55887]

ChIP-Exo-Seq composite graph for Anti-POLR3D (NBP2-55887) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for POLR3D Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR3D

RNA polymerase III subunit D (POLR3D) is one of 17 subunits of the RNA polymerase III (PolIII) which synthesizes small RNAs, such as 5S rRNA and tRNA. POLR3D include RNA polymerase III subunit C4, RPC4, DNA-directed RNA polymerase III subunit D, DNA-directed RNA polymerase III 47 kDa polypeptide, RPC53, protein BN51, TSBN51, and BN51T.

Alternate Names

BN51, BN51 (BHK21) temperature sensitivity complementing, BN51TDNA-directed RNA polymerase III 47 kDa polypeptide, DNA-directed RNA polymerase III subunit D, DNA-directed RNA polymerase III subunit RPC4, polymerase (RNA) III (DNA directed) polypeptide D, 44kDa, Protein BN51, RNA polymerase III 47 kDa subunit, RNA polymerase III subunit C4, RPC4, RPC53 homolog, temperature sensitive complementation, cell cycle specific, tsBN51, TSBN51RPC53

Gene Symbol

POLR3D

Additional POLR3D Products

Product Documents for POLR3D Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POLR3D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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