Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all POLR3A Antibodies »

Product Specifications

Immunogen

polymerase(RNA) III(DNA directed) polypeptide A, 155kDa. (Uniprot# O14802)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR3A Antibody - BSA Free

POLR3A Antibody - BSA Free

Western Blot: POLR3A Antibody - BSA Free [NBP3-45691] -

Western Blot: POLR3A Antibody - BSA Free [NBP3-45691] - MCF7 cells were subjected to SDS PAGE followed by western blot with (POLR3A antibody) at dilution of 1:300

Applications for POLR3A Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000
Application Notes
Theoretical Molecular Weight is 140-150 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLR3A

RNA polymerase III (Pol III) is a multi-subunit complex responsible for catalyzing the transcription of DNA into RNA. POLR3A (polymerase (RNA) III (DNA directed) polypeptide A), also known as RPC1 or RPC155, is a 1,390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase beta chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman's syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria.

Alternate Names

DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase III subunit A, DNA-directed RNA polymerase III subunit RPC1, EC 2.7.7.6, hRPC155, POL3A, polymerase (RNA) III (DNA directed) polypeptide A, 155kDa, RNA polymerase III 155 kDa subunit, RNA polymerase III subunit C160, RNA polymerase III subunit RPC155-D, RPC1, RPC155RNA polymerase III subunit C1

Gene Symbol

POLR3A

Additional POLR3A Products

Product Documents for POLR3A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR3A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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