POLR2D Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92280
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit POLR2D Antibody - BSA Free (NBP1-92280) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for POLR2D Antibody - BSA Free
![Immunohistochemistry-Paraffin: POLR2D Antibody [NBP1-92280]](https://resources.bio-techne.com/images/products/POLR2D-Antibody-Immunohistochemistry-Paraffin-NBP1-92280-img0004.jpg "Immunohistochemistry-Paraffin: POLR2D Antibody [NBP1-92280]")
Immunohistochemistry-Paraffin: POLR2D Antibody [NBP1-92280]
Immunohistochemistry-Paraffin: POLR2D Antibody [NBP1-92280] - Staining of human cervix, uterine shows nuclear positivity in squamous epithelial cells.![POLR2D Antibody - BSA Free Western Blot: POLR2D Antibody - BSA Free [NBP1-92280]](https://resources.bio-techne.com/images/products/nbp1-92280_rabbit-polyclonal-polr2d-antibody-295202516123918.jpg "Western Blot: POLR2D Antibody - BSA Free [NBP1-92280]")
Western Blot: POLR2D Antibody - BSA Free [NBP1-92280]
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
![POLR2D Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: POLR2D Antibody - BSA Free [NBP1-92280]](https://resources.bio-techne.com/images/products/nbp1-92280_rabbit-polyclonal-polr2d-antibody-25620259103154.jpg "Chromatin Immunoprecipitation-exo-Seq: POLR2D Antibody - BSA Free [NBP1-92280]")
Chromatin Immunoprecipitation-exo-Seq: POLR2D Antibody - BSA Free [NBP1-92280]
ChIP-Exo-Seq composite graph for Anti-POLR2D (NBP1-92280) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for POLR2D Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: POLR2D
Alternate Names
DNA-directed RNA polymerase II 16 kDa polypeptide, DNA-directed RNA polymerase II subunit D, DNA-directed RNA polymerase II subunit RPB4, HSRBP4, HSRPB4, polymerase (RNA) II (DNA directed) polypeptide D, RBP4, RNA polymerase II 16 kDa subunit, RNA polymerase II subunit B4, RNA polymerase II subunit hsRBP4, RPB16
Gene Symbol
POLR2D
Additional POLR2D Products
Product Documents for POLR2D Antibody - BSA Free
Product Specific Notices for POLR2D Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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