POLR1C Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87114
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (95%), Rat (93%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:NNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQFRLQVRCTRNPHAAKDSSDPNELYVNHKVYTRHMTWIPLGNQADLFPEGTIRPVHD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for POLR1C Antibody - BSA Free
![Western Blot: POLR1C Antibody [NBP1-87114]](https://resources.bio-techne.com/images/products/POLR1C-Antibody-Western-Blot-NBP1-87114-img0005.jpg "Western Blot: POLR1C Antibody [NBP1-87114]")
Western Blot: POLR1C Antibody [NBP1-87114]
Western Blot: POLR1C Antibody [NBP1-87114] - Analysis in control (vector only transfected HEK293T lysate) and POLR1C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: POLR1C Antibody [NBP1-87114]](https://resources.bio-techne.com/images/products/POLR1C-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87114-img0004.jpg "Immunocytochemistry/ Immunofluorescence: POLR1C Antibody [NBP1-87114]")
Immunocytochemistry/ Immunofluorescence: POLR1C Antibody [NBP1-87114]
Immunocytochemistry/Immunofluorescence: POLR1C Antibody [NBP1-87114] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.![POLR1C Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: POLR1C Antibody - BSA Free [NBP1-87114]](https://resources.bio-techne.com/images/products/nbp1-87114_rabbit-polyclonal-polr1c-antibody-25620259121974.jpg "Chromatin Immunoprecipitation-exo-Seq: POLR1C Antibody - BSA Free [NBP1-87114]")
Chromatin Immunoprecipitation-exo-Seq: POLR1C Antibody - BSA Free [NBP1-87114]
ChIP-Exo-Seq composite graph for Anti-POLR1C (NBP1-87114) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for POLR1C Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: POLR1C
Alternate Names
AC40, DNA-directed RNA polymerase I subunit C, DNA-directed RNA polymerases I and III 40 kDa polypeptide, POLR1E, polymerase (RNA) I polypeptide C, 30kDa, RNA polymerase I subunit, RPA39RNA polymerases I and III subunit AC1, RPA40TCS3, RPA5RPAC1DNA-directed RNA polymerases I and III subunit RPAC1, RPC40
Gene Symbol
POLR1C
Additional POLR1C Products
Product Documents for POLR1C Antibody - BSA Free
Product Specific Notices for POLR1C Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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