Pollen allergen Phl p 1 Antibody (Clone 25) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81276
Recombinant Monoclonal Antibody
![ELISA: Pollen allergen Phl p 1 Antibody (Clone 25) - Chimeric - Azide and BSA Free [NBP2-81276]](https://resources.bio-techne.com/images/products/Pollen-allergen-Phl-p-1-Antibody-Clone-25-Chimeric-ELISA-NBP2-81276-img0001.jpg "ELISA: Pollen allergen Phl p 1 Antibody (Clone 25) - Chimeric - Azide and BSA Free [NBP2-81276]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Plant
Applications
Western Blot, ELISA, Dot Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # Clone 25
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant Phl p 1, a grass pollen allergen.
Reactivity Notes
Expected to react with Timothy-Grass (Phleum pratense).
Specificity
This antibody binds to the Timothy grass allergen Phl p 1 and shows cotss reactivity with natural group 1 allergens from a variety of grass species such as oat, sweet vernal grass, Bermuda grass, rye grass, common reed, Kentucky blue grass, rye, wheat and maize (weak).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Novus Biologicals Rabbit Pollen allergen Phl p 1 Antibody (Clone 25) - Chimeric - Azide and BSA Free (NBP2-81276) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images
ELISA: Pollen allergen Phl p 1 Antibody (Clone 25) - Chimeric - Azide and BSA Free [NBP2-81276]
ELISA: Pollen allergen Phl p 1 Antibody (Clone 25) - Chimeric [NBP2-81276] - Binding curve of [NBP2-81276] to Phl p 1 ELISA Plate coated with Phl p 1 at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-81276. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used.Applications
Application
Recommended Usage
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to Phl p 1 and competes with up to 43.4% (mean 19.8%) of IgE from allergic patient serum.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Pollen allergen Phl p 1
Alternate Names
Allergen Phl p I, grass pollen allergen
UniProt
Additional Pollen allergen Phl p 1 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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