Podoplanin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90211

Novus Biologicals, part of Bio-Techne
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NBP1-90211
NBP1-90211-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Podoplanin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RLPRVWEARAPSLSGAPAPTPPAPPPSRSSRLGLWPRCFLIFPQLRILLLGPQESNNSTGTMWKVSALLFVLGSASLWVLAEGASTGQPEDDTETTGLEGGVAMPGAEDDVVTPGTSEDRYKSGLTTLVATSVNS

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26030524).

Marker

Lymphatic Endothelium Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Podoplanin Antibody - BSA Free (NBP1-90211) is a polyclonal antibody validated for use in IHC and WB. Anti-Podoplanin Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Podoplanin Antibody - BSA Free

Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211]

Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211]

Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining in human placenta and liver tissues . Corresponding PDPN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211]

Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211]

Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining of human placenta shows moderate membranous positivity in endothelial cells.
Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211]

Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211]

Immunohistochemistry-Paraffin: Podoplanin Antibody [NBP1-90211] - Staining of human liver shows no positivity in hepatocytes as expected.
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Applications for Podoplanin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

Additional Podoplanin Products

Product Documents for Podoplanin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Podoplanin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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