PMM2/Phosphomannomutase 2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00351

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PMM2/Phosphomannomutase 2 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human PMM2/Phosphomannomutase 2 (Accession#: O15305; Met1-Ser246)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PMM2/Phosphomannomutase 2 Antibody - Azide and BSA Free

PMM2/Phosphomannomutase 2 Antibody - Azide and BSA Free

Immunoprecipitation: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] -

Immunoprecipitation: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - PMM2 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 uL NBP3-00351 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00351, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 27 kDa Observed band size: 27 kDa
PMM2/Phosphomannomutase 2 Antibody - Azide and BSA Free

Western Blot: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] -

Western Blot: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - NBP3-00351 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.)

Applications for PMM2/Phosphomannomutase 2 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PMM2/Phosphomannomutase 2

PMM2, also known as Phosphomannomutase 2, is a 246 amino acid protein that is 28 kDa, catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate, which is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. Studies are being performed on the relationship of this protein to congenital disorder of glycosylation, hydrops fetalis, premature ovarian failure, cerebellar hypoplasia, metabolic disorders, alcohol abuse, intellectual disability, hypertrophic cardiomyopathy, cerebellar ataxia, galactosemia, peripheral neuropathy, hypogonadism, neuropathy, hypotonia, cardiomyopathy, thrombocytopenia, ataxia, alcoholism, and malaria. The PMM2 protein has also shown an interaction with HIST1H4A, HIST1H4B, HIST1H4D, HIST1H4E, HIST1H4C, and over 130 other proteins in synthesis of substrates in N-glycan biosynthesis, asparagine N-linked glycosylation, metabolism of proteins, post-translational protein modification, biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein, fructose and mannose metabolism, amino sugar and nucleotide sugar metabolism, GDP-mannose biosynthesis, and colanic acid building blocks biosynthesis pathways.

Alternate Names

CDG1, CDG1a, CDGS, EC 5.4.2.8, phosphomannomutase 2, PMM 2

Gene Symbol

PMM2

Additional PMM2/Phosphomannomutase 2 Products

Product Documents for PMM2/Phosphomannomutase 2 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PMM2/Phosphomannomutase 2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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