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PML Protein Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-59788

Novus Biologicals, part of Bio-Techne
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NB100-59788

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 575 and 625 of promyelocytic leukemia using the numbering given in entry NP_150241.2 (GeneID 5371).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PML Protein Antibody

Western Blot: PML Protein Antibody [NB100-59788]

Western Blot: PML Protein Antibody [NB100-59788]

Western Blot: PML Protein Antibody [NB100-59788] - Detection of Human PML on HeLa whole cell lysate using NB100-59788. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59787 and another PML antibody.
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59788]

Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59788]

Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59788] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for PML Protein Antibody

Application
Recommended Usage

Immunohistochemistry

1:200- 1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PML

PML is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. PML is a multifunctional protein involved in activities such as tumor growth suppression, hematopoietic differentiation, gene transcription, apoptotic induction, and viral immunity. PML is often involved in chromosomal translocations with the retinoic acid receptor alpha gene. PML-retinoic acid receptor translocations are associated with acute promyelocytic leukemia.

Long Name

Promyelocytic Leukemia

Alternate Names

MYL, RNF71, TRIM19

Entrez Gene IDs

5371 (Human)

Gene Symbol

PML

UniProt

Additional PML Products

Product Documents for PML Protein Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PML Protein Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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