PLAP Antibody (MSVA-350R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57232
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # MSVA-350R
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human ALPP protein Swissprot P05187
Localization
Cell Surface and Cytoplasmic
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.
Positive Control
Postive control:Placenta (mature): A strong membranous and cytoplasmic staining of cytotrophoblast and syncytiotrophoblast must be seen..
Negative Control
Negitve control:Appendix: PLAP immunostaining must be absent in all structures.
Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.
Applications for PLAP Antibody (MSVA-350R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Preservative
<0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: PLAP
Alternate Names
DOA1, FLJ12699, phospholipase A-2-activating protein, phospholipase A2-activating protein, PLAP
Gene Symbol
PLAA
Additional PLAP Products
Product Specific Notices for PLAP Antibody (MSVA-350R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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