PLA2R1 Antibody (CL0474) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52933

Novus Biologicals, part of Bio-Techne
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NBP2-52933-25ul
NBP2-52933
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Conjugate
Catalog #
Alexa Fluor 350
NBP3-43909AF350
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Alexa Fluor 405
NBP3-43909AF405
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Alexa Fluor 488
NBP3-43909AF488
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Alexa Fluor 532
NBP3-43909AF532
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Alexa Fluor 594
NBP3-43909AF594
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Alexa Fluor 647
NBP3-43909AF647
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Alexa Fluor 700
NBP3-43909AF700
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Alexa Fluor 750
NBP3-43909AF750
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Alexa Fluor Plus 488
NBP3-43909AFP488
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Alexa Fluor Plus 555
NBP3-43909AFP555
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Alexa Fluor Plus 594
NBP3-43909AFP594
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Alexa Fluor Plus 647
NBP3-43909AFP647
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Alexa Fluor Plus 680
NBP3-43909AFP680
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Allophycocyanin
NBP3-43909APC
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Biotin
NBP3-43909B
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CoraFluor 1
NBP3-43909CL1
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DyLight 350
NBP3-43909UV
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DyLight 405
NBP3-43909V
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DyLight 488
NBP3-43909G
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DyLight 550
NBP3-43909R
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DyLight 594
NBP3-43909DL594
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DyLight 650
NBP3-43909C
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DyLight 680
NBP3-43909FR
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DyLight 755
NBP3-43909IR
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FITC
NBP3-43909F
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HRP
NBP3-43909H
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Janelia Fluor 525
NBP3-43909JF525
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Janelia Fluor 549
NBP3-43909JF549
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Janelia Fluor 646
NBP3-43909JF646
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Janelia Fluor 669
NBP3-43909JF669
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mFluor Violet 450 SE
NBP3-43909MFV450
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mFluor Violet 500 SE
NBP3-43909MFV500
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mFluor Violet 610 SE
NBP3-43909MFV610
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PE
NBP3-43909PE
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PerCP
NBP3-43909PCP
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0474

Format

BSA Free

Concentration

1 mg/ml

View all PLA2R1 Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q13018, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PLA2R1 Antibody (CL0474) - BSA Free

Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933]

Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933]

Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933] - Staining in human kidney and pancreas tissues. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
Western Blot: PLA2R1 Antibody (CL0474) [NBP2-52933]

Western Blot: PLA2R1 Antibody (CL0474) [NBP2-52933]

Western Blot: PLA2R1 Antibody (CL0474) [NBP2-52933] - Analysis in human kidney tissue.
Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (CL0474) [NBP2-52933]

Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (CL0474) [NBP2-52933]

Immunocytochemistry/Immunofluorescence: PLA2R1 Antibody (CL0474) [NBP2-52933] - Enhanced validation of the Anti-PLA2R1 monoclonal antibody by comparing the immunofluorescence staining in human cell lines Hela and MCF7 exhibiting a relatively high and low expression of PLA2R1 (based on RNA-seq values), respectively. The PLA2R1 (green signal) is present in Hela cells but is absent in MCF7 cells. The anti-HDAC1 antibody was used as a loading control (red signal). Antibody staining is shown in green.
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Applications for PLA2R1 Antibody (CL0474) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20000 - 1:50000

Immunohistochemistry-Paraffin

1:20000 -1:50000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLA2R1

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 (PubMed 7721806)).(supplied by OMIM)

Long Name

Phospholipase A2 Receptor 1, 180kDa

Alternate Names

CLEC13C, PLA2G1R

Gene Symbol

PLA2R1

Additional PLA2R1 Products

Product Documents for PLA2R1 Antibody (CL0474) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PLA2R1 Antibody (CL0474) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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