PKR Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77266
![Western Blot: PKR AntibodyBSA Free [NBP1-77266]](https://resources.bio-techne.com/images/products/PKR-Antibody-Western-Blot-NBP1-77266-img0008.jpg "Western Blot: PKR AntibodyBSA Free [NBP1-77266]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PKR. The immunogen is located within the last 50 amino acids of PKR. Amino Acid Squence: TVWKKSPEKNERHT
Specificity
Human PKR has 2 isoforms, including isoform 1 (551aa, 62.1kD) and isoform S (510aa, 57.4kD. This antibody detects both human isoforms. Mouse PKR has 1 isoform (515aa, 58.3kD). Rat PKR has only one isoform identified so far (513aa, 58.3kD).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit PKR Antibody - BSA Free (NBP1-77266) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-PKR Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PKR Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: PKR Antibody - BSA Free [NBP1-77266] -
Immunocytochemistry/ Immunofluorescence: PKR Antibody - BSA Free [NBP1-77266] - Validation of PKR in Mouse Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).![Western Blot: PKR AntibodyBSA Free [NBP1-77266]](https://resources.bio-techne.com/images/products/PKR-Antibody-Western-Blot-NBP1-77266-img0009.jpg "Western Blot: PKR AntibodyBSA Free [NBP1-77266]")
Western Blot: PKR AntibodyBSA Free [NBP1-77266]
Western Blot: PKR Antibody [NBP1-77266] - WB Validation of PKR in Human Cell Lines. Antibodies: PKR. Secondary: Goat anti-rabbit IgG HRP conjugate.
Applications for PKR Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2.5-5 ug/ml
Immunohistochemistry-Paraffin
2.5-5 ug/ml
Western Blot
1 ug/ml
Reviewed Applications
Read 1 review rated 5 using NBP1-77266 in the following applications:
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PKR
Long Name
Protein Kinase R
Alternate Names
EIF2AK2, P68 Kinase, PPP1R83, PRKR, Protein Kinase R
Gene Symbol
EIF2AK2
UniProt
Additional PKR Products
Product Documents for PKR Antibody - BSA Free
Product Specific Notices for PKR Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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