PKC iota/lambda/zeta Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # AF4465CL1
![PKC iota/lambda/zeta Antibody [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/af4465cl1_sheep-pkc-iota-lambda-zeta-pab-corafluor-1-12920251459170.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
CoraFluor 1
Antibody Source
Polyclonal Sheep IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
E. coli-derived recombinant human PKC iota
Ile455-Val596
Accession # P41743
Ile455-Val596
Accession # P41743
Specificity
Detects human, mouse and rat PKC iota/ lambda/ zeta in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for PKC iota/lambda/zeta Antibody [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for PKC iota/lambda/zeta Antibody [CoraFluor™ 1]
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: PKC iota/lambda/zeta
Atypical members of the Protein Kinase C (PKC) family of serine/threonine protein kinases include PKC iota/lambda and PKC zeta. Members of the atypical PKC subfamily are calcium-independent and do not require phosphatidylserine, diacylglycerol (DAG), or exogenous phorbol esters for activation. They have been shown to serve as convergent downstream targets for the PI 3-kinase and TC10 signaling pathways and can regulate NF-kappa B activation during inflammation and cell survival signaling. Atypical PKCs are also implicated in insulin signaling and glucose regulation.
Long Name
Protein Kinase C iota/lambda/zeta
Additional PKC iota/lambda/zeta Products
Product Documents for PKC iota/lambda/zeta Antibody [CoraFluor™ 1]
Product Specific Notices for PKC iota/lambda/zeta Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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