Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PINX1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 225 to 275 of human PIN2 Interacting Protein 1 using the numbering given in entry NP_060354.4 (GeneID 54984).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PINX1 Antibody - BSA Free

Western Blot: PINX1 Antibody [NBP2-32265]

Western Blot: PINX1 Antibody [NBP2-32265]

Western Blot: PINX1 Antibody [NBP2-32265] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PINX1 antibody NBP2-32265 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: PINX1 Antibody [NBP2-32265]

Immunohistochemistry: PINX1 Antibody [NBP2-32265]

Immunohistochemistry: PINX1 Antibody [NBP2-32265] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PINX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: PINX1 Antibody [NBP2-32265]

Immunoprecipitation: PINX1 Antibody [NBP2-32265]

Immunoprecipitation: PINX1 Antibody [NBP2-32265] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PINX1 antibody NBP2-32265 used for IP at 6 ug per reaction. PINX1 was also immunoprecipitated by rabbit anti-PINX1 antibody BL15294. For blotting immunoprecipitated PINX1, NBP2-32265 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for PINX1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PINX1

PINX1 is a protein that contains a TID (telomerase inhibiting domain) domain which is capable of binding MCRS1, TERT and TERF1. PINX1 has been shown to be a potent telomerase inhibitor and putative tumor suppressor.

Alternate Names

FLJ20565, hepatocellular carcinoma-related putative tumor suppressor, Liver-related putative tumor suppressor, LPTLPinX1, LPTS67-11-3 protein, MGC8850, PIN2 interacting protein 1, PIN2/TERF1 interacting, telomerase inhibitor 1, PIN2/TERF1-interacting telomerase inhibitor 1, PIN2-interacting protein 1, Pin2-interacting protein X1, Protein 67-11-3, TRF1-interacting protein 1

Gene Symbol

PINX1

UniProt

NP_060354.4 (Human)

Additional PINX1 Products

Product Documents for PINX1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PINX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.