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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig

Cited:

Human, Mouse, Rat, Guinea Pig

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for PIEZO2 Antibody - BSA Free

Immunogen

A synthetic peptide made to an internal portion of the human PIEZO2 protein (between residues 1600-1650) [UniProt# Q9H5I5]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34335288) Guinea Pig reactivity reported in scientific literature (PMID: 30324494).

Localization

Membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIEZO2 Antibody - BSA Free

Immunofluorescent Staining of PIEZO2 in Mouse Neurons

Immunofluorescent Staining of PIEZO2 in Mouse Neurons

PIEZO2 expression and mechanical characterization of MTN neurons in Piezo2cKO mice. Coronal brainstem sections of MTN neurons with DAPI stained nuclei (blue) that were immunolabeled for PIEZO2 (red, left) or GFP (green, middle: parvalbumin-positive cell) and the merged image of both. In WT littermates mice PIEZO2 was detected in the 23 GFP+ MTM neurons (top) and PIEZO2 was detected in 7 MTM neurons of the 20 that expressed GFP in Pvalb-Cre:RCE:Piezo2cKO mice (bottom; arrowhead, PIEZO2+ neuron). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep25923) licensed under a CC-BY license.
Immunohistochemical Analysis of PIEZO2 in Paraffin Embedded Mouse Dorsal Root Ganglion

Immunohistochemical Analysis of PIEZO2 in Paraffin Embedded Mouse Dorsal Root Ganglion

Analysis of PIEZO2 in dorsal root ganglion of embryonic day (E) 13.5 mouse. PIEZO2 in red, DAPI in blue. Antigen retrieval with 1x DAKO antigen retrieval solution, by heating in a microwave oven, for total time of 6 minutes 10 seconds. Washing buffer 1 x PBS with 0.1% Triton X-100. Blocking in 5% donkey serum (Jackson lab) in washing buffer. Primary antibody diluted 1:1000, incubation 18 hours in room temperature. Secondary antibody donkey anti-rabbit Cy3, 1:400, 3 hours in room temperature. Image from verified customer review.
Immunocytochemistry/Immunofluorescence Staining of PIEZO2 in A431 Cells

Immunocytochemistry/Immunofluorescence Staining of PIEZO2 in A431 Cells

Immunocytochemistry/Immunofluorescence: PIEZO2 Antibody [NBP1-78624] - PIEZO2 antibody was tested at 1:50 in A431 cells with FITC (green) .Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Applications for PIEZO2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

2 ug/ml. Use reported in scientific literature (PMID 31791130)
Application Notes
In ICC/IF, membrane staining was observed in A431 cells. In paraffin sections, membrane and cytoplasmic staining is seen in mouse epidermis tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-78624 in the following applications:

Published Applications

Read 26 publications using NBP1-78624 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIEZO2

Piezo2 (FAM38B) is a mechanosensitive ion channel which responds to mechanically activated currents in bladder, colon, lung, and dorsal root ganglia. This suggests Piezo2 is important for somatasensory mechanotransduction including touch and pain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins, and are very large in size.

Long Name

PIEZO2

Alternate Names

C18orf30, C18orf58, DA3, DA5, DAIPT, FAM38B, FAM38B2, family with sequence similarity 38, member A pseud, family with sequence similarity 38, member B2, HsT748, HsT771, MWKS, piezo-type mechanosensitive ion channel component

Entrez Gene IDs

63895 (Human)

Gene Symbol

Q9H5I5

UniProt

Additional PIEZO2 Products

Product Documents for PIEZO2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIEZO2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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