PI 3-Kinase p85 beta Antibody (24GB5210)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50834

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NBP3-50834-100ul
NBP3-50834-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB5210

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PI 3-Kinase p85 beta Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PI 3-Kinase p85 beta (Uniprot: O00459)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PI 3-Kinase p85 beta Antibody (24GB5210)

PI 3-Kinase p85 beta Antibody (24GB5210)

Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 beta Antibody (24GB5210)[NBP3-50834] -

Immunocytochemical staining of HepG2 cells with PI 3-Kinase p85 beta antibody (1:1,000) . Nuclei were stained blue with DAPI; PI 3-Kinase p85 beta was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
PI 3-Kinase p85 beta Antibody (24GB5210)

Flow Cytometry: PI 3-Kinase p85 beta Antibody (24GB5210)[NBP3-50834] -

Flow cytometric analysis of PI 3-Kinase p85 betaexpression in HepG2 cells using PI 3-Kinase p85 beta antibody (1:2,000). Green, isotype control; red, PI3 Kinase p85 beta.
PI 3-Kinase p85 beta Antibody (24GB5210)

Western Blot: PI 3-Kinase p85 beta Antibody (24GB5210)[NBP3-50834] -

Western blotting analysis using PI 3-Kinase p85 beta antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for PI 3-Kinase p85 beta Antibody (24GB5210)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PI 3-Kinase p85 beta

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 beta). p85 beta is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 beta is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Long Name

Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta

Alternate Names

p85-beta, PI 3Kinase p85 beta, PIK3R2

Gene Symbol

PIK3R2

Additional PI 3-Kinase p85 beta Products

Product Documents for PI 3-Kinase p85 beta Antibody (24GB5210)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PI 3-Kinase p85 beta Antibody (24GB5210)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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