Phospho-STAT6 (Y641) PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC37173P

Recombinant Monoclonal Antibody.
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IC37173P-025
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Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1248D

View all STAT6 Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human STAT6 Y641 site.
Accession # P42226

Specificity

Detects human STAT6 when phosphorylated at Y641.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-STAT6 (Y641) PE-conjugated Antibody

Detection of STAT6 antibody in Human peripheral blood mononuclear cells (PBMCs) antibody by Flow Cytometry.

Detection of STAT6 in Human peripheral blood mononuclear cells (PBMCs) by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) Th2-stimulated or (B) unstimulated were stained with Rabbit Anti-Human Phospho-STAT6 (Y641) PE-conjugated Monoclonal Antibody (Catalog # IC37173P) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC1051P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Applications for Phospho-STAT6 (Y641) PE-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Th2-stimulated human peripheral blood mononuclear cells (PBMCs) fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.

Long Name

Signal Transducer and Activator of Transcription 6

Alternate Names

D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT

Entrez Gene IDs

6778 (Human); 20852 (Mouse); 362896 (Rat)

Gene Symbol

STAT6

UniProt

P42226

Additional STAT6 Products

Product Documents for Phospho-STAT6 (Y641) PE-conjugated Antibody

Product Datasheets

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Product Specific Notices for Phospho-STAT6 (Y641) PE-conjugated Antibody

For research use only

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