PHLDB2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36546
![Western Blot: PHLDB2 Antibody [NBP2-36546]](https://resources.bio-techne.com/images/products/PHLDB2-Antibody-Western-Blot-NBP2-36546-img0001.jpg "Western Blot: PHLDB2 Antibody [NBP2-36546]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immungoen recognized by the antibody maps to a region between residue 250 to 300 of human LL5beta using the numbering given in entry NP_001127910.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PHLDB2 Antibody - BSA Free
Western Blot: PHLDB2 Antibody [NBP2-36546]
Western Blot: PHLDB2 Antibody [NBP2-36546] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LL5beta antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes![Immunoprecipitation: PHLDB2 Antibody [NBP2-36546]](https://resources.bio-techne.com/images/products/PHLDB2-Antibody-Immunoprecipitation-NBP2-36546-img0002.jpg "Immunoprecipitation: PHLDB2 Antibody [NBP2-36546]")
Immunoprecipitation: PHLDB2 Antibody [NBP2-36546]
Immunoprecipitation: PHLDB2 Antibody [NBP2-36546] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated LL5beta, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for PHLDB2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PHLDB2
Alternate Names
pleckstrin homology-like domain, family B, member 2
Gene Symbol
PHLDB2
UniProt
Additional PHLDB2 Products
Product Documents for PHLDB2 Antibody - BSA Free
Product Specific Notices for PHLDB2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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