PHGDH Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32201
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-32201-img0008.jpg "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (97%), Chimpanzee (100%), Porcine (97%). Backed by our 100% Guarantee.
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Knockout (KO) Validated Rabbit PHGDH Antibody - BSA Free (NBP1-32201) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PHGDH Antibody - BSA Free
![Knockout Validated: PHGDH Antibody [NBP1-32201]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Western-Blot-NBP1-32201-img0013.jpg "Western Blot: PHGDH Antibody [NBP1-32201]")
Western Blot: PHGDH Antibody [NBP1-32201]
Western Blot: PHGDH Antibody [NBP1-32201] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Western Blot: PHGDH Antibody [NBP1-32201]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Western-Blot-NBP1-32201-img0007.jpg "Western Blot: PHGDH Antibody [NBP1-32201]")
Western Blot: PHGDH Antibody [NBP1-32201]
Western Blot: PHGDH Antibody [NBP1-32201] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-32201]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32201-img0014.jpg "Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-32201]")
Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-32201]
Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - PHGDH antibody detects PHGDH protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody diluted at 1:250. Blue: Fluoroshield with DAPI.![Western Blot: PHGDH Antibody [NBP1-32201]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Western-Blot-NBP1-32201-img0006.jpg "Western Blot: PHGDH Antibody [NBP1-32201]")
![Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-32201]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32201-img0012.jpg "Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-32201]")
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-32201-img0005.jpg "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201]")
![Immunoprecipitation: PHGDH Antibody [NBP1-32201]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Immunoprecipitation-NBP1-32201-img0011.jpg "Immunoprecipitation: PHGDH Antibody [NBP1-32201]")
Applications for PHGDH Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PHGDH
Long Name
3-Phosphoglycerate Dehydrogenase
Alternate Names
3PGDH, PDGH3
Entrez Gene IDs
26227 (Human)
Gene Symbol
PHGDH
UniProt
Additional PHGDH Products
Product Documents for PHGDH Antibody - BSA Free
Product Specific Notices for PHGDH Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.