PHGDH Antibody (CL0555) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52939
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0555
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from O43175, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PHGDH Antibody (CL0555) - BSA Free
Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] -
Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4![Western Blot: PHGDH Antibody (CL0555) [NBP2-52939]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-CL0555-Western-Blot-NBP2-52939-img0007.jpg "Western Blot: PHGDH Antibody (CL0555) [NBP2-52939]")
Western Blot: PHGDH Antibody (CL0555) [NBP2-52939]
Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos
![PHGDH Antibody (CL0555) - BSA Free Immunocytochemistry/ Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939] -](https://resources.bio-techne.com/images/products/nbp2-52939_-immunocytochemistry-immunofluorescence-639174076994246571.jpg "Immunocytochemistry/ Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939] -")
Applications for PHGDH Antibody (CL0555) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHGDH
Long Name
3-Phosphoglycerate Dehydrogenase
Alternate Names
3PGDH, PDGH3
Gene Symbol
PHGDH
Additional PHGDH Products
Product Documents for PHGDH Antibody (CL0555) - BSA Free
Product Specific Notices for PHGDH Antibody (CL0555) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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