PGK1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19784
![Western Blot: PGK1 Antibody [NBP2-19784]](https://resources.bio-techne.com/images/products/PGK1-Antibody-Western-Blot-NBP2-19784-img0013.jpg "Western Blot: PGK1 Antibody [NBP2-19784]")
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Yeast
Predicted:
Bovine (96%), Chimpanzee (100%), Porcine (96%), Rhesus Macaque (100%), Sheep (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%), Xenopus laevis (84%), Chicken (88%).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit PGK1 Antibody - BSA Free (NBP2-19784) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-PGK1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PGK1 Antibody - BSA Free
![Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784]](https://resources.bio-techne.com/images/products/PGK1-Antibody-Immunohistochemistry-Paraffin-NBP2-19784-img0008.jpg "Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784]")
Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784]
Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.![Western Blot: PGK1 Antibody [NBP2-19784]](https://resources.bio-techne.com/images/products/PGK1-Antibody-Western-Blot-NBP2-19784-img0010.jpg "Western Blot: PGK1 Antibody [NBP2-19784]")
Western Blot: PGK1 Antibody [NBP2-19784]
Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein by Western blot analysis. 50 ug Rat liver lysate/extract, 10% SDS-PAGE, PGK1 antibody dilution: 1:1000.![Western Blot: PGK1 Antibody [NBP2-19784]](https://resources.bio-techne.com/images/products/PGK1-Antibody-Western-Blot-NBP2-19784-img0011.jpg "Western Blot: PGK1 Antibody [NBP2-19784]")
Western Blot: PGK1 Antibody [NBP2-19784]
Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein in 1% O2-treated samples by Western blot analysis. Upper panel: PGK1 antibody. Lower panel: beta Actin antibody. A: 30 ug HeLa whole cell lysate/extract (untreated). B: 30 ug HeLa whole cell lysate/extract (1% O2 treatment for 24 hours).![Immunocytochemistry/ Immunofluorescence: PGK1 Antibody [NBP2-19784]](https://resources.bio-techne.com/images/products/PGK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19784-img0007.jpg "Immunocytochemistry/ Immunofluorescence: PGK1 Antibody [NBP2-19784]")
Applications for PGK1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
Assay dependent
Proximity Ligation Assay
Assay dependent
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 5 using NBP2-19784 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PGK1
Long Name
Phosphoglycerate Kinase-1
Alternate Names
MIG10, PGKA, PRP2
Gene Symbol
PGK1
UniProt
Additional PGK1 Products
Product Specific Notices for PGK1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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