Skip to main content

PGC1 alpha Antibody

Catalog # NB100-60955 | Novus Biologicals a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
NB100-60955

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for PGC1 alpha Antibody

Immunogen

Peptide with sequence C-DGLFDDSEDESDK, from the internal region of the protein sequence according to NP_037393.1; NP_001317680.1; NP_001317681.1; NP_001317682.1; NP_001341756.1.

Epitope

DGLFDDSEDESDK

Predicted Species

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29020797).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGC1 alpha Antibody

PGC1 alpha Antibody

Flow Cytometry: PGC1 alpha Antibody [NB100-60955] -

Flow Cytometry: PGC1 alpha Antibody [NB100-60955] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
PGC1 alpha Antibody

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-60955] -

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NB100-60955] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
PGC1 alpha Antibody

Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NB100-60955] -

Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NB100-60955] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for PGC1 alpha Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/uL

Immunohistochemistry-Paraffin

6-8 ug/ml

Peptide ELISA

Detection limit 1:2000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Preliminary testing showed a band at approx 90kDa in nuclear lysates of cell lines A431 and HEK293 after 0.3-1ug/ml antibody staining (calculated MW of 91.0kDa according to Human NP_037393.1). Primary incubation 1 hour at room temperature.
Use in IHC-P reported in scientific literature (PMID: 23763823). Use in IHC reported in scientific literature (PMID: 22227854).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB100-60955 in the following applications:

Published Applications

Read 8 publications using NB100-60955 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PGC1 alpha

Peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1alpha), PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha (theoretical molecular weight 91 kDa) belongs to a family of transcription coactivators which includes two other proteins; PGC-1beta and PGC-1-related coactivators. PGC1 alpha interacts with various transcription factors (e.g., FOXO1, PPAR-(alpha, delta and gamma), LXR and FXR), promoting the transactivation of their specific target genes (1). As a transcription coactivator, PGC1 alpha is found in the nucleus where it interacts with transcriptional activators and chromatin modifiers such as p300/CBP and SRC-1 to induce targeted gene expression (1, 2). Transcription factors which interact with PGC1 alpha are involved in several functions related to cellular energy homeostasis including mitochondrial biogenesis, glucose and fatty acid metabolism, and skeletal muscle remodeling. Upstream regulators of PGC1 alpha activity include proteins that play a central role as energy sensors including SIRT1 and AMPK which, through different mechanisms, induce PGC1 alpha activity (3).

PGC1 alpha is highly expressed in skeletal muscle, cardiac muscle, and brown adipose tissue, all tissues with high oxidative capacity (1, 2). PGC1 alpha is also induced by energy taxing physiological states, for example increased physical activity, reduced body temperature, and reduced food intake (3). Because of its role in pathways controlling cellular energy expenditure, PGC1 alpha dysfunction has been associated with pathophysiological conditions such as type 2 diabetes and the metabolic syndrome (3).

References

1.Liang, H., & Ward, W. F. (2006). PGC-1alpha: A key regulator of energy metabolism. American Journal of Physiology - Advances in Physiology Education. https://doi.org/10.1152/advan.00052.2006

2.Arany, Z., He, H., Lin, J., Hoyer, K., Handschin, C., Toka, O., ... Spiegelman, B. M. (2005). Transcriptional coactivator PGC-1alpha controls the energy state and contractile function of cardiac muscle. Cell Metabolism. https://doi.org/10.1016/j.cmet.2005.03.002

3.Canto, C., & Auwerx, J. (2009). PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Current Opinion in Lipidology. https://doi.org/10.1097/MOL.0b013e328328d0a4

Long Name

Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha

Alternate Names

LEM6, PPARGC1A

Entrez Gene IDs

10891 (Human); 19017 (Mouse); 83516 (Rat)

Gene Symbol

PPARGC1A

Product Documents for PGC1 alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PGC1 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...

FAQs for PGC1 alpha Antibody

Showing  1 - 2 of 2 FAQs Showing All
  • Q: My question is whether this antibody (PGC1 alpha Antibody) detects the phosphorylated PGC1 alpha protein? Or if it just detects the total PGC1 alpha protein?

    A: NB100-60955 is not specific for a particular phosphorylated form of PGC1 alpha. This protein is only phosphorylated on two residues but has 13 known acetylation sites, which is why the protein is detected at around 100 kDa instead of the predicted 91 kDa. This antibody should detect the unphosphorylated and unacetylated form as well, if they are present in your sample.

  • Q: I wanted information on your primary antibody for PGC1 alpha NB100-60955, whether it detects the phosphorylated PGC1 alpha protein, or if it just detects the total PGC1 alpha protein.

    A: NB100-60955 is not specific for a particular phosphorylated form of PGC1 alpha. This protein is only phosphorylated on two residues but has 13 known acetylation sites, which is why the protein is detected at around 100 kDa instead of the predicted 91 kDa. This antibody should detect the unphosphorylated and unacetylated form as well, if they are present in your sample.

Showing  1 - 2 of 2 FAQs Showing All
Loading...