Perforin Antibody (rPRF1/8058) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24297
Recombinant Monoclonal Antibody
![Perforin Antibody (rPRF1/8058) - Azide and BSA Free Western Blot: Perforin Antibody (rPRF1/8058) - Azide and BSA Free [NBP3-24297] -](https://resources.bio-techne.com/images/products/nbp3-24297_mouse-perforin-mab-rprf1-8058-azide-and-bsa-free-2412025801619.jpg "Western Blot: Perforin Antibody (rPRF1/8058) - Azide and BSA Free [NBP3-24297] -")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # rPRF1/8058
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human Perforin protein fragment (around aa 355-555) (exact sequence is proprietary)
Localization
Cytoplasm.
Marker
Apoptosis Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Prepared in 10mM PBS WITHOUT BSA & azide.
Antibody without azide - store at -20 to -80C
Antibody without azide - store at -20 to -80C
Scientific Data Images for Perforin Antibody (rPRF1/8058) - Azide and BSA Free
Western Blot: Perforin Antibody (rPRF1/8058) - Azide and BSA Free [NBP3-24297] -
Western Blot: Perforin Antibody (rPRF1/8058) - Azide and BSA Free [NBP3-24297] - Western blot analysis of HDLM-2 cell lysate using Perforin Antibody (rPRF1/8058)![Perforin Antibody (rPRF1/8058) - Azide and BSA Free Immunohistochemistry-Paraffin: Perforin Antibody (rPRF1/8058) - Azide and BSA Free [NBP3-24297] -](https://resources.bio-techne.com/images/products/nbp3-24297_mouse-perforin-mab-rprf1-8058-azide-and-bsa-free-24120257595020.jpg "Immunohistochemistry-Paraffin: Perforin Antibody (rPRF1/8058) - Azide and BSA Free [NBP3-24297] -")
Immunohistochemistry-Paraffin: Perforin Antibody (rPRF1/8058) - Azide and BSA Free [NBP3-24297] -
Immunohistochemistry-Paraffin: Perforin Antibody (rPRF1/8058) - Azide and BSA Free [NBP3-24297] - Formalin-fixed, paraffin-embedded human spleen stained with Perforin Antibody (rPRF1/8058). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Perforin Antibody (rPRF1/8058) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Western Blot
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Perforin
Long Name
Perforin 1 (Pore Forming Protein)
Alternate Names
Cytolysin, FLH2, HPLH2, P1, PFP, PRF1
Gene Symbol
PRF1
Additional Perforin Products
Product Documents for Perforin Antibody (rPRF1/8058) - Azide and BSA Free
Product Specific Notices for Perforin Antibody (rPRF1/8058) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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