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PER1 Antibody

Catalog # NBP2-24589 | Novus Biologicals a Bio-Techne Brand
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NBP2-24589
NBP2-24589SS

Key Product Details

Species Reactivity

Human, Mouse, Bovine, Canine, Equine, Primate

Applications

Immunoblotting, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Summary for PER1 Antibody

Immunogen

A portion of amino acids 1150-1200 of human PER1 was used as the immunogen.

Predicted Species

Rat (93%). Backed by our 100% Guarantee.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29937374).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PER1 Antibody

Western Blot: PER1 Antibody [NBP2-24589] - Analysis of PER1 in HEPG2 cell lysates in the 1) absence and 2) presence of immunizing peptide using NBP2-24589. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: PER1 Antibody [NBP2-24589] - Human brain tissue using an isotype control (top) and NBP2-24589 (bottom) at 5 ug/ml.

Applications for PER1 Antibody

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 29937374)

Immunohistochemistry-Paraffin

5 ug/ml

Knockout Validated

reported in scientific literature (Hamilton et al)

Western Blot

5-10 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 1.5 using NBP2-24589 in the following applications:

Published Applications

Read 3 publications using NBP2-24589 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PER1

Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.

Long Name

Period Circadian Protein 1

Alternate Names

PERIOD1, RIGUI

Gene Symbol

PER1

Product Documents for PER1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PER1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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