PEPP2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37696
![Western Blot: PEPP2 Antibody [NBP2-37696]](https://resources.bio-techne.com/images/products/PEPP2-Antibody-Western-Blot-NBP2-37696-img0001.jpg "Western Blot: PEPP2 Antibody [NBP2-37696]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody maps to a region between residue 876 to 925 of human Pleckstrin Homology Domain Containing, Family A Member 6 using the numbering given in entry NP_061885.3 (GeneID 54477).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PEPP2 Antibody - BSA Free (NBP2-37696) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PEPP2 Antibody - BSA Free
Western Blot: PEPP2 Antibody [NBP2-37696]
Western Blot: PEPP2 Antibody [NBP2-37696] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PLEKHA5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: PEPP2 Antibody [NBP2-37696]](https://resources.bio-techne.com/images/products/PEPP2-Antibody-Immunoprecipitation-NBP2-37696-img0002.jpg "Immunoprecipitation: PEPP2 Antibody [NBP2-37696]")
Immunoprecipitation: PEPP2 Antibody [NBP2-37696]
Immunoprecipitation: PEPP2 Antibody [NBP2-37696] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PLEKHA5 antibody used for IP at 6 ug per reaction. PLEKHA5 was also immunoprecipitated by rabbit anti-PLEKHA5 antibody. For blotting immunoprecipitated PLEKHA5, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: PEPP2 Antibody [NBP2-37696]](https://resources.bio-techne.com/images/products/PEPP2-Antibody-Immunoprecipitation-NBP2-37696-img0003.jpg "Immunoprecipitation: PEPP2 Antibody [NBP2-37696]")
Immunoprecipitation: PEPP2 Antibody [NBP2-37696]
Immunoprecipitation: PEPP2 Antibody [NBP2-37696] - Detection of human PLEKHA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLEKHA5 antibody NBP2-37696 used for IP at 6 ug per reaction. PLEKHA5 was also immunoprecipitated by rabbit anti-PLEKHA5 antibody NBP2-37695. For blotting immunoprecipitated PLEKHA5, NBP2-37696 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for PEPP2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PEPP2
Alternate Names
FLJ10667, FLJ26734, KIAA1686FLJ31492, PEPP-2, PEPP2phosphoinositol 3-phosphate-binding protein-2, PH domain-containing family A member 5, Phosphoinositol 3-phosphate-binding protein 2, pleckstrin homology domain containing, family A member 5, pleckstrin homology domain-containing family A member 5
Gene Symbol
PLEKHA5
UniProt
Additional PEPP2 Products
Product Documents for PEPP2 Antibody - BSA Free
Product Specific Notices for PEPP2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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