Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PEPP2 Antibodies »

Product Specifications

Immunogen

This antibody maps to a region between residue 876 to 925 of human Pleckstrin Homology Domain Containing, Family A Member 6 using the numbering given in entry NP_061885.3 (GeneID 54477).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PEPP2 Antibody - BSA Free

Western Blot: PEPP2 Antibody [NBP2-37696]

Western Blot: PEPP2 Antibody [NBP2-37696]

Western Blot: PEPP2 Antibody [NBP2-37696] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PLEKHA5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PEPP2 Antibody [NBP2-37696]

Immunoprecipitation: PEPP2 Antibody [NBP2-37696]

Immunoprecipitation: PEPP2 Antibody [NBP2-37696] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PLEKHA5 antibody used for IP at 6 ug per reaction. PLEKHA5 was also immunoprecipitated by rabbit anti-PLEKHA5 antibody. For blotting immunoprecipitated PLEKHA5, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PEPP2 Antibody [NBP2-37696]

Immunoprecipitation: PEPP2 Antibody [NBP2-37696]

Immunoprecipitation: PEPP2 Antibody [NBP2-37696] - Detection of human PLEKHA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLEKHA5 antibody NBP2-37696 used for IP at 6 ug per reaction. PLEKHA5 was also immunoprecipitated by rabbit anti-PLEKHA5 antibody NBP2-37695. For blotting immunoprecipitated PLEKHA5, NBP2-37696 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PEPP2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PEPP2

PEPP2 is a member of the homeobox PEPP subfamily. PEPP2 is highly expressed in heart and kidney, and also in reproductive tissues; the human homolog is specifically expressed in testis.

Alternate Names

FLJ10667, FLJ26734, KIAA1686FLJ31492, PEPP-2, PEPP2phosphoinositol 3-phosphate-binding protein-2, PH domain-containing family A member 5, Phosphoinositol 3-phosphate-binding protein 2, pleckstrin homology domain containing, family A member 5, pleckstrin homology domain-containing family A member 5

Gene Symbol

PLEKHA5

UniProt

NP_061885.3 (Human)

Additional PEPP2 Products

Product Documents for PEPP2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PEPP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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