PCNA Antibody (PC10) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB500-106B
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Chicken, Drosophila, Fish, Primate, Rabbit, Yeast, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # PC10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Protein A-rat PCNA fusion obtained from pC2T
Reactivity Notes
Fish reactivity reported in scientific literature (PMID: 30075024).
Specificity
Specific for PCNA p36 protein expressed at high levels in proliferating cells.
Marker
Proliferation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Applications for PCNA Antibody (PC10) [Biotin]
Application
Recommended Usage
Chromatin Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry Free-Floating
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This PCNA (PC10) antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation, Immunocytochemistry/Immunofluorescence (PMID: 22068968) Flow Cytometry and Western blot. It detects a band at 30 kDa by Western blot. For IHC on paraffin sections heat-mediated citrate buffer antigen retrieval is recommended.ELISA was reported in scientific literature.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: PCNA
Long Name
Proliferating Cell Nuclear Antigen
Alternate Names
cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
Gene Symbol
PCNA
Additional PCNA Products
Product Documents for PCNA Antibody (PC10) [Biotin]
Product Specific Notices for PCNA Antibody (PC10) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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