Parkin Antibody (PRK8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29838
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PRK8
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Full length recombinant human Parkin. Epitope has been mapped to the second ring domain (aa 399-465).
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
Parkin52kDa.May be observed as a doublet at 52 and 46kDa weight may be detected.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
Relevant Entrez Gene Numbers: NM_004562
Scientific Data Images for Parkin Antibody (PRK8)
Western Blot: Parkin Antibody (PRK8) [NBP2-29838]
Western Blot: Parkin Antibody (PRK8) [NBP2-29838] - HEK293 cell lysate was probed with anti- Parkin, clone PRK8 (1:2000 dilution). Arrow indicates Parkin (~52kDa).Western Blot: Parkin Antibody (PRK8) [NBP2-29838] -
Immunoblotting analysis of mitophagy proteins. (A) Representative immunoblots for control HT22 cells and mTau-HT22 cells with or without Rlip overexpression and silencing. (B) Represents quantitative densitometry analysis of mitophagy proteins. Pink 1 and Parkin levels were significantly decreased in Mutant Tau when compared with the control. Increased Pink 1 and Parkin levels were observed in Rlip-cDNA with control cells. Decreased Pink 1 and Parkin levels were observed in Tau + Rlip (siRNA) when compared with control HT22 cells. However, significantly increased Pink and Parkin levels were observed in mutant Tau + Rlip-cDNA when compared with the control. (* p = 0.05, ** p = 0.005, *** p = 0.001, and **** p = 0.0001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37371116), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Parkin Antibody (PRK8) [NBP2-29838] -
Immunoblotting analysis of mitochondrial mitophagy and synaptic proteins. (A) Representative immunoblots for untreated and hAbKI mice and treated hAbKI Urolithin A and EGCG. (B) Quantitative-densitometry analysis of PINK1, Parkin, synaptophysin and PSD95, which shows significant reduction in the hAbKI mice compared to the treated hAbKI mice Urolithin A and EGCG. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36078067), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Parkin Antibody (PRK8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Reviewed Applications
Read 1 review rated 1 using NBP2-29838 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites, containing 30% glycerol.
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Parkin
Long Name
Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1
Alternate Names
AR-JP, PARK2, PDJ, PRKN
Gene Symbol
PRKN
UniProt
Additional Parkin Products
Product Specific Notices for Parkin Antibody (PRK8)
Relevant Entrez Gene Numbers: NM_004562
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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