PARG1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12573
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human PARG1 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for PARG1 Antibody - BSA Free
![Western Blot: PARG1 Antibody [NBP3-12573]](https://resources.bio-techne.com/images/products/PARG1-Antibody-Western-Blot-NBP3-12573-img0001.jpg "Western Blot: PARG1 Antibody [NBP3-12573]")
Western Blot: PARG1 Antibody [NBP3-12573]
Western Blot: PARG1 Antibody [NBP3-12573] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:142 kDaObserved band size:170 kDa![Immunoprecipitation: PARG1 Antibody [NBP3-12573]](https://resources.bio-techne.com/images/products/PARG1-Antibody-Immunoprecipitation-NBP3-12573-img0003.jpg "Immunoprecipitation: PARG1 Antibody [NBP3-12573]")
Immunoprecipitation: PARG1 Antibody [NBP3-12573]
Immunoprecipitation: PARG1 Antibody [NBP3-12573] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-ARHGAP29 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ARHGAP29 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 142 kDaObserved band size :171 kDa
Western Blot: PARG1 Antibody - BSA Free [NBP3-12573] -
Western Blot: PARG1 Antibody - BSA Free [NBP3-12573] - NBP3-12573 at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:142 kDa Observed band size:170 kDaApplications for PARG1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PARG1
Alternate Names
PARG1PTPL1-associated RhoGAP 1 (PARG1), PTPL1-associated RhoGAP protein 1, Rho GTPase activating protein 29, rho GTPase-activating protein 29, Rho-type GTPase-activating protein 29, RP11-255E17.1
Gene Symbol
ARHGAP29
Additional PARG1 Products
Product Specific Notices for PARG1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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