Pappalysin-1/PAPP-A Antibody (9) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05116
![Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]](https://resources.bio-techne.com/images/products/Pappalysin-1-PAPP-A-Antibody-NA-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05116-img0002.jpg "Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 9
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Purified human PAPP-A
Epitope
The epitope is located on the PAPP-A subunit of the heterotetramer.
Specificity
Specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Concentration for any given batch will be between 0.85 - 1.15 mg/mL.
Scientific Data Images for Pappalysin-1/PAPP-A Antibody (9) - BSA Free
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in paraffin embedded human placenta tissue.![Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]](https://resources.bio-techne.com/images/products/Pappalysin-1-PAPP-A-Antibody-NA-BSA-Free-Immunohistochemistry-Frozen-NBP1-05116-img0001.jpg "Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]")
Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]
Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in frozen human placenta tissue.Applications for Pappalysin-1/PAPP-A Antibody (9) - BSA Free
Application
Recommended Usage
ELISA
1:8000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
ELISA: In sandwich ELISA the optimal combination is NBP1-05116 used as coating antibody and biotinylated NBP1-05043 used as detection antibody. NBP1-05116 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat.
WB: NBP1-05116 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of NBP2-21614 and NBP1-05043.
IHC: NBP1-05116 can be used in IHC.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Pappalysin-1/PAPP-A
Long Name
Pregnancy-associated Plasma Protein 1
Alternate Names
ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A1, PAPPA, Pappalysin1
Gene Symbol
PAPPA
Additional Pappalysin-1/PAPP-A Products
Product Documents for Pappalysin-1/PAPP-A Antibody (9) - BSA Free
Product Specific Notices for Pappalysin-1/PAPP-A Antibody (9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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