Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PAK1 interacting protein 1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 290 and 340 of human PAK/PLC-interacting protein 1 (WD repeat protein 84) using the numbering given in entry NP_060376.2 (GeneID 55003).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK1 interacting protein 1 Antibody - BSA Free

Western Blot: PAK1 interacting protein 1 Antibody [NB100-77292]

Western Blot: PAK1 interacting protein 1 Antibody [NB100-77292]

Western Blot: PAK1 interacting protein 1 Antibody [NB100-77292] - Detection of Human PIP1/WDR84 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PIP1/WDR84 antibody NB100-77292 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: PAK1 interacting protein 1 Antibody [NB100-77292]

Western Blot: PAK1 interacting protein 1 Antibody [NB100-77292]

Western Blot: PAK1 interacting protein 1 Antibody [NB100-77292] - Detection of Human PIP1/WDR84 on HeLa whole cell lysate using NB100-77292. PIP1/WDR84 was also immunoprecipitated by rabbit anti-PIP1/WDR84 antibody NB100-77293.

Applications for PAK1 interacting protein 1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PAK1 interacting protein 1

PIP1/WDR84 (PAK1IP1) was identified as a p21-activated protein kinase (PAK) interacting protein that functions as a negative regulator of PAK activity and PAK signaling pathways. PIP1/WDR84 (PAK1IP1) contains 5 G beta-like WD repeats. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation.

Alternate Names

bA421M1.5, FLJ20624, hPIP1WD repeat-containing protein 84, MAK11, p21-activated protein kinase-interacting protein 1, PAK1 interacting protein 1, PIP1PAK/PLC-interacting protein 1, RP11-421M1.5, WDR84PAK1-interacting protein 1

Entrez Gene IDs

55003 (Human)

Gene Symbol

PAK1IP1

UniProt

NP_060376.2 (Human)

Additional PAK1 interacting protein 1 Products

Product Documents for PAK1 interacting protein 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PAK1 interacting protein 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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