PABPN1 Antibody (7V4X8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15539
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7V4X8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PABPN1 (Q86U42). MAAAAAAAAAAGAAGGRGSGPGRRRHLVPGAGGEAGEGAPGGAGDYGNGLESEELEPEELLLEPEPEPEPEEEPPRPRAPPGAPGPGPGSGAPGSQEEEE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PABPN1 Antibody (7V4X8)
![Western Blot: PABPN1 Antibody (7V4X8) [NBP3-15539]](https://resources.bio-techne.com/images/products/PABPN1-Antibody-7V4X8-Western-Blot-NBP3-15539-img0007.jpg "Western Blot: PABPN1 Antibody (7V4X8) [NBP3-15539]")
Western Blot: PABPN1 Antibody (7V4X8) [NBP3-15539]
Western Blot: PABPN1 Antibody (7V4X8) [NBP3-15539] - Western blot analysis of extracts of various cell lines, using PABPN1 Rabbit mAb (NBP3-15539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: PABPN1 Antibody (7V4X8) [PABPN1] -
Immunocytochemistry/ Immunofluorescence: PABPN1 Antibody (7V4X8) [PABPN1] - Confocal imaging of C6 cells using PABPN1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) .The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunohistochemistry: PABPN1 Antibody (7V4X8) [PABPN1] -
Immunohistochemistry: PABPN1 Antibody (7V4X8) [PABPN1] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PABPN1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Applications for PABPN1 Antibody (7V4X8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PABPN1
Alternate Names
Nuclear poly(A)-binding protein 1, OPMD, PAB2poly(A) binding protein II, PABII, PABP-2, PABP2polyadenylate-binding protein 2, poly(A) binding protein 2, poly(A) binding protein, nuclear 1, Poly(A)-binding protein 2, Poly(A)-binding protein II, poly(A)-binding protein, nuclear 1, Polyadenylate-binding nuclear protein 1
Gene Symbol
PABPN1
Additional PABPN1 Products
Product Documents for PABPN1 Antibody (7V4X8)
Product Specific Notices for PABPN1 Antibody (7V4X8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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