PA28 Activator alpha Subunit/PSME1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19991
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (96%), Mouse (94%), Porcine (98%), Rat (95%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human PA28 Activator alpha Subunit/PSME1. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit PA28 Activator alpha Subunit/PSME1 Antibody (NBP2-19991) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PA28 Activator alpha Subunit/PSME1 Antibody
Immunocytochemistry/ Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991] -
PSME1 antibody detects PSME1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSME1 protein stained by PSME1 antibody (NBP2-19991) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991] -
Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME1 antibody (NBP2-19991) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for PA28 Activator alpha Subunit/PSME1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PA28 Activator alpha Subunit
Long Name
Proteasome (Prosome, Macropain) Activator Subunit 1
Alternate Names
11S Regulator Complex Subunit alpha, IFI5111, PSME1, REG alpha
Gene Symbol
PSME1
UniProt
Additional PA28 Activator alpha Subunit Products
Product Documents for PA28 Activator alpha Subunit/PSME1 Antibody
Product Specific Notices for PA28 Activator alpha Subunit/PSME1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.