p53 [p Ser46] Antibody (GT1018) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13598
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # GT1018
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser46 of human p53. The exact sequence is proprietary.
Modification
p Ser 46
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Centrifuge briefly prior to opening.
Scientific Data Images for p53 [p Ser46] Antibody (GT1018) - Azide and BSA Free
Western Blot: p53 [p Ser46] Antibody (GT1018) [NBP3-13598] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser46) antibody [GT1018] (NBP3-13598) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: p53 [p Ser46] Antibody (GT1018) [NBP3-13598] - p53 (phospho Ser46) antibody [GT1018] detects p53 (phospho Ser46) protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p53 (phospho Ser46) protein stained by p53 (phospho Ser46) antibody [GT1018] (NBP3-13598) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: p53 [p Ser46] Antibody (GT1018) [NBP3-13598] - p53 (phospho Ser46) antibody [GT1018] detects p53 (phospho Ser46) protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. p53 (phospho Ser46) stained by p53 (phospho Ser46) antibody [GT1018] (NBP3-13598) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for p53 [p Ser46] Antibody (GT1018) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: p53
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
Additional p53 Products
Product Documents for p53 [p Ser46] Antibody (GT1018) - Azide and BSA Free
Product Specific Notices for p53 [p Ser46] Antibody (GT1018) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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