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p400 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55019

Novus Biologicals, part of Bio-Techne
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NBP2-55019
NBP2-55019-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GTIKTSVTGTSMPTGAVSGNVIVNTIAGVPAATFQSINKRLASPVAPGALTTPGGSAPAQVVHTQPPPRAVGSPATATPDLVSMATTQGVRAVTSVTASAVVTTNLTPVQTPARSLVPQVSQAT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit p400 Antibody - BSA Free (NBP2-55019) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for p400 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: p400 Antibody [NBP2-55019]

Immunocytochemistry/ Immunofluorescence: p400 Antibody [NBP2-55019]

Immunocytochemistry/Immunofluorescence: p400 Antibody [NBP2-55019] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
p400 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: p400 Antibody - BSA Free [NBP2-55019]

Chromatin Immunoprecipitation-exo-Seq: p400 Antibody - BSA Free [NBP2-55019]

ChIP-Exo-Seq composite graph for Anti-EP400 (NBP2-55019) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for p400 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p400

P400 protein is a 250 kd glycoprotein, characteristic of the cerebellum, which is accumulated at the endoplasmic reticulum, at the plasma membrane and at the post-synaptic density of Purkinje cells.

Alternate Names

CAG repeat protein 32, CAGH32hDomino, DKFZP434I225, Domino homolog, E1A binding protein p400, E1A-binding protein p400, EC 3.6.1, EC 3.6.4.-, FLJ45115, KIAA1498p400 kDa SWI2/SNF2-related protein, KIAA1818, P400, p400 SWI2/SNF2-related protein, TNRC12FLJ42018, trinucleotide repeat containing 12, Trinucleotide repeat-containing gene 12 protein

Gene Symbol

EP400

Additional p400 Products

Product Documents for p400 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p400 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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