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P2X3/P2RX3 Antibody (HL3347)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48793

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-48793

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3347

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human P2X3/P2RX3. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit P2X3/P2RX3 Antibody (HL3347) (NBP3-48793) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for P2X3/P2RX3 Antibody (HL3347)

P2X3/P2RX3 Antibody (HL3347)

P2X3/P2RX3 Antibody (HL3347)-NBP3-48793

Paraffin-embedded mouse testis (left) and mouse liver (right).P2X3 stained by P2X3 antibody [HL3347] (diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
P2X3/P2RX3 Antibody (HL3347)

P2X3/P2RX3 Antibody (HL3347)-NBP3-48793

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2X3 antibody [HL3347] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for P2X3/P2RX3 Antibody (HL3347)

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000
Application Notes
IHC-P ,Antigen retrieval: Citrate buffer (pH 6.0) is suitable, while Tris-EDTA (pH9.0) is not recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P2X3/P2RX3

P2RX3 is a member of the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and transduces ATP-evoked nociceptor activation. P2RX3 is important for peripheral pain responses, and controls urinary bladder volume reflexes.

Long Name

P2X Purinergic Receptor 3

Alternate Names

P2RX3

Gene Symbol

P2RX3

Additional P2X3/P2RX3 Products

Product Documents for P2X3/P2RX3 Antibody (HL3347)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for P2X3/P2RX3 Antibody (HL3347)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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