p27/Kip1 Antibody (SX53G8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32889
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) [NBP2-32889]](https://resources.bio-techne.com/images/products/p27-Kip1-Antibody-SX53G8-Immunohistochemistry-Paraffin-NBP2-32889-img0002.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) [NBP2-32889]")
![Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (SX53G8) [NBP2-32889]](https://resources.bio-techne.com/images/products/p27-Kip1-Antibody-SX53G8-Immunocytochemistry-Immunofluorescence-NBP2-32889-img0005.jpg "Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (SX53G8) [NBP2-32889]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SX53G8
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Purified GST-p27 fusion protein of human origin (Uniprot: P46527)
Localization
Nuclear
Specificity
This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for p27/Kip1 Antibody (SX53G8)
Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (SX53G8) [NBP2-32889]
Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (SX53G8) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) [NBP2-32889]](https://resources.bio-techne.com/images/products/p27-Kip1-Antibody-SX53G8-Immunohistochemistry-Paraffin-NBP2-32889-img0001.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) [NBP2-32889]")
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) [NBP2-32889]
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Formalin-paraffin human colon stained with p27 MAb (SX53G8)![Flow Cytometry: p27/Kip1 Antibody (SX53G8) [NBP2-32889]](https://resources.bio-techne.com/images/products/p27-Kip1-Antibody-SX53G8-Flow-Cytometry-NBP2-32889-img0007.jpg "Flow Cytometry: p27/Kip1 Antibody (SX53G8) [NBP2-32889]")
Flow Cytometry: p27/Kip1 Antibody (SX53G8) [NBP2-32889]
Flow Cytometry: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Flow Cytometry of human p27/Kip1 on HeLa cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled p27/Kip1 Mouse Monoclonal Antibody (SX53G8).![Flow Cytometry: p27/Kip1 Antibody (SX53G8) [NBP2-32889]](https://resources.bio-techne.com/images/products/p27-Kip1-Antibody-SX53G8-Flow-Cytometry-NBP2-32889-img0006.jpg "Flow Cytometry: p27/Kip1 Antibody (SX53G8) [NBP2-32889]")
![p27/Kip1 Antibody (SX53G8) Western Blot: p27/Kip1 Antibody (SX53G8) [NBP2-32889] -](https://resources.bio-techne.com/images/products/nbp2-32889_mouse-monoclonal-p27-kip1-antibody-sx53g8-295202516354544.jpeg "Western Blot: p27/Kip1 Antibody (SX53G8) [NBP2-32889] -")
![p27/Kip1 Antibody (SX53G8) Western Blot: p27/Kip1 Antibody (SX53G8) [NBP2-32889] -](https://resources.bio-techne.com/images/products/nbp2-32889_mouse-monoclonal-p27-kip1-antibody-sx53g8-295202516375618.jpeg "Western Blot: p27/Kip1 Antibody (SX53G8) [NBP2-32889] -")
Applications for p27/Kip1 Antibody (SX53G8)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
0.25-0.5 ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: p27/Kip1
Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Alternate Names
CDKN1B, Kip1
Gene Symbol
CDKN1B
UniProt
Additional p27/Kip1 Products
Product Documents for p27/Kip1 Antibody (SX53G8)
Product Specific Notices for p27/Kip1 Antibody (SX53G8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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