p19ARF/CDKN2A Antibody (5-C3-1) [PE]
Novus Biologicals, part of Bio-Techne | Catalog # NB200-174PE
![p19ARF/CDKN2A Antibody (5-C3-1) [PE]](https://resources.bio-techne.com/images/products/nb200-174pe_rat-monoclonal-p19arf-cdkn2a-antibody-5-c3-1-pe-41220241042107.png "Flow Cytometry: p19ARF/CDKN2A Antibody (5-C3-1) [PE]")
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Golden Syrian Hamster (Negative)
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 5-C3-1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide containing amino acids 54-75 of the murine p19ARF/CDKN2A. [UniProt# Q64364]
Epitope
The epitope recognized is between amino acids 62-75 of murine p19ARF/CDKN2A.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 27177758). The antibody does not react with Golden Syrian hamster. Armenian hamster has not been tested.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for p19ARF/CDKN2A Antibody (5-C3-1) [PE]
Flow Cytometry: p19ARF/CDKN2A Antibody (5-C3-1) [PE]
Flow Cytometry: p19ARF/CDKN2A Antibody (5-C3-1) [PE] - Conducted the antibody trial with two different dilutions (1:500 and 1:100) and compared them to an IgG control which was also PE labelled. Image from a verified customer review.Applications for p19ARF/CDKN2A Antibody (5-C3-1) [PE]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Knockout Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB200-174. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Reviewed Applications
Read 1 review rated 5 using NB200-174PE in the following applications:
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Plan Your Experiments
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Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: p19ARF/CDKN2A
Alternate Names
alternative reading frame, ARF, ARF-INK4a, CDK4I, CDKN2A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4), cyclin-dependent kinase inhibitor 2A, isoform 3, cyclin-dependent kinase inhibitor 2A, isoforms 1/2, cyclin-dependent kinase inhibitor protein, INK4, INK4a, Ink4a/Arf, INK4a-ARF, mitochondrial smARF', MTS1, p14, p14ARF, P16, p16(INK4a), p16-INK4, P16INK4A, p16-INK4a, p19, p19, p19ARF, Pctr1
Gene Symbol
Cdkn2a
Additional p19ARF/CDKN2A Products
Product Documents for p19ARF/CDKN2A Antibody (5-C3-1) [PE]
Product Specific Notices for p19ARF/CDKN2A Antibody (5-C3-1) [PE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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