p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-45125
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 30859-S17
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
BALB/c mice were immunized with recombinant p16INK4a/CDKN2A (Uniprot#: P42771)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Scientific Data Images for p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free
Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free [NBP3-45125] -
Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free [NBP3-45125] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-45125 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45125, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.Applications for p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The binding specificity and affinity of this antibody for p16 was determined by ELISA. Western blotting experiments suggested that this antibody is capable of recognizing p16 and tumor cells expressing p16 molecules like Hela cells.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: p16INK4a / CDKN2A
Long Name
p16 Cyclin Dependent Kinase 4 Inhibitor 2A
Alternate Names
ARF, CDK4I, CDKN2A, INK4a, MLM, p14ARF, p16, p19ARF
Gene Symbol
CDKN2A
Additional p16INK4a / CDKN2A Products
Product Documents for p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free
Product Specific Notices for p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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