OSBPL11 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36544
![Western Blot: OSBPL11 Antibody [NBP2-36544]](https://resources.bio-techne.com/images/products/OSBPL11-Antibody-Western-Blot-NBP2-36544-img0001.jpg "Western Blot: OSBPL11 Antibody [NBP2-36544]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 1 to 50 of human Oxysterol Binding Protein-Like 11 using the numbering given in entry NP_073613.2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for OSBPL11 Antibody - BSA Free
Western Blot: OSBPL11 Antibody [NBP2-36544]
Western Blot: OSBPL11 Antibody [NBP2-36544] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: OSBPL11 Antibody [NBP2-36544]](https://resources.bio-techne.com/images/products/OSBPL11-Antibody-Immunoprecipitation-NBP2-36544-img0002.jpg "Immunoprecipitation: OSBPL11 Antibody [NBP2-36544]")
Immunoprecipitation: OSBPL11 Antibody [NBP2-36544]
Immunoprecipitation: OSBPL11 Antibody [NBP2-36544] - Detection of human ORP11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP11 antibody NBP2-36544 used for IP at 6 ug per reaction. ORP11 was also immunoprecipitated by rabbit anti-ORP11 antibody NBP2-36545. For blotting immunoprecipitated ORP11, NBP2-36544 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for OSBPL11 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1: 10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: OSBPL11
Alternate Names
FLJ13012, FLJ13164, ORP11OSBP-related protein 11, ORP-11TCCCIA00292, OSBP12, oxysterol binding protein-like 11, oxysterol-binding protein-related protein 11
Gene Symbol
OSBPL11
UniProt
Additional OSBPL11 Products
Product Documents for OSBPL11 Antibody - BSA Free
Product Specific Notices for OSBPL11 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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