This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ORP150/HSP12A Antibody (201) - Azide and BSA Free

Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890]

Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:111 kDa Observed band size:170 kDa

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of human HSP12A in human cirrhosis with rabbit monoclonal antibody (1:200).

Immunoprecipitation: ORP150/HSP12A Antibody (201) [NBP2-89890]

Immunoprecipitation: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate 2 uL anti-HSP12A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-HSP12A rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa

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Applications for ORP150/HSP12A Antibody (201) - Azide and BSA Free

Application

Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ORP150/HSP12A

HYOU1 is encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Long Name

Oxygen Regulated Protein, 150 kDa

Alternate Names

HSP12A, HYOU1

Gene Symbol

HYOU1

Additional ORP150/HSP12A Products

Product Documents for ORP150/HSP12A Antibody (201) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for ORP150/HSP12A Antibody (201) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

ORP150/HSP12A Antibody (201) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89890

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Format

Concentration

View all ORP150/HSP12A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Description

Scientific Data Images for ORP150/HSP12A Antibody (201) - Azide and BSA Free

Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890]

Immunoprecipitation: ORP150/HSP12A Antibody (201) [NBP2-89890]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] -

Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody (201) [NBP2-89890] -

Applications for ORP150/HSP12A Antibody (201) - Azide and BSA Free

ELISA

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: ORP150/HSP12A

Long Name

Alternate Names

Gene Symbol

Additional ORP150/HSP12A Products

Product Documents for ORP150/HSP12A Antibody (201) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for ORP150/HSP12A Antibody (201) - Azide and BSA Free