Oct-4A Antibody (03) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12775
![Flow Cytometry: Oct-4A Antibody (03) [NBP3-12775]](https://resources.bio-techne.com/images/products/Oct-4A-Antibody-03-Flow-Cytometry-NBP3-12775-img0001.jpg "Flow Cytometry: Oct-4A Antibody (03) [NBP3-12775]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 03
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to the C-terminus of Human Oct-4A.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Oct-4A Antibody (03) - Azide and BSA Free
Flow Cytometry: Oct-4A Antibody (03) [NBP3-12775]
Flow Cytometry: Oct-4A Antibody (03) [NBP3-12775] - Analysis of Oct4 in human embryonic stem (ES) cells. Human embryonic stem (ES) cells were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization buffer and stained with Purified Mouse anti-Oct4 monoclonal antibody, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Applications for Oct-4A Antibody (03) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:25-1:100
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Oct-4A
Oct-3/4, alternately Oct-3 or Oct-4, is POU5F1 (POU domain containing, class 5, transcription factor 1), a 360 amino acid (aa) transcription factor that is expressed in totipotent embryonic stem and germ cells. The human Oct-4, Oct-3/4 or POU5F1 gene can be transcribed into at least three transcripts (Oct-4A, Oct-4B, and Oct-4B1) and generates four protein isoforms by alternative splicing and alternative translation initiation. Oct-4A expression is restricted to embryonic stem (ES) and embryonic carcinoma (EC) cells and is believed to be the transcription factor responsible for the pluripotency properties of embryonic stem (ES) cells. In contrast, Oct-4B/4B1 can be detected in various nonpluripotent cell types and cannot sustain ES cell pluripotency and self-renewal.
Long Name
Octamer-binding Transcription Factor 4A
Alternate Names
Oct4A
Gene Symbol
POU5F1
Additional Oct-4A Products
Product Documents for Oct-4A Antibody (03) - Azide and BSA Free
Product Specific Notices for Oct-4A Antibody (03) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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