Occludin Antibody (CL1567) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52972
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1567-Immunohistochemistry-Paraffin-NBP2-52972-img0001.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]")
![Western Blot: Occludin Antibody (CL1567) [NBP2-52972]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1567-Western-Blot-NBP2-52972-img0008.jpg "Western Blot: Occludin Antibody (CL1567) [NBP2-52972]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL1567
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q16625, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Occludin Antibody (CL1567) - BSA Free
Western Blot: Occludin Antibody (CL1567) [NBP2-52972]
Western Blot: Occludin Antibody (CL1567) [NBP2-52972] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: OCLN Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)![Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1567) [NBP2-52972]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1567-Immunocytochemistry-Immunofluorescence-NBP2-52972-img0009.jpg "Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1567) [NBP2-52972]")
Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1567) [NBP2-52972]
Immunocytochemistry/Immunofluorescence: Occludin Antibody (CL1567) [NBP2-52972] - Staining of CACO-2 cells using the Anti-OCLN monoclonal antibody, showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1567-Immunohistochemistry-Paraffin-NBP2-52972-img0007.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]")
Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]
Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972] - Staining of human tonsil shows membranous immunoreactivity in squamous epithelium cells.![Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1567-Immunohistochemistry-Paraffin-NBP2-52972-img0002.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1567-Immunohistochemistry-Paraffin-NBP2-52972-img0003.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1567-Immunohistochemistry-Paraffin-NBP2-52972-img0004.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]](https://resources.bio-techne.com/images/products/Occludin-Antibody-CL1567-Immunohistochemistry-Paraffin-NBP2-52972-img0005.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972]")
Applications for Occludin Antibody (CL1567) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Occludin
Alternate Names
BLCPMG, OCLN
Gene Symbol
OCLN
Additional Occludin Products
Product Documents for Occludin Antibody (CL1567) - BSA Free
Product Specific Notices for Occludin Antibody (CL1567) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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