O-GlcNAcase/OGA/MGEA5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32233
![Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233]](https://resources.bio-techne.com/images/products/O-GlcNAcase-OGA-MGEA5-Antibody-Immunohistochemistry-NBP2-32233-img0006.jpg "Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Cited:
Western Blot, Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 500 to 550 of human Meningioma Expressed Antigen 5 using the numbering given in entry NP_036347.1 (GeneID 10724).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for O-GlcNAcase/OGA/MGEA5 Antibody - BSA Free
Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233]
Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Section of human testicular seminoma. Antibody: Affinity purified rabbit anti- MGEA5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).![Immunoprecipitation: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233]](https://resources.bio-techne.com/images/products/O-GlcNAcase-OGA-MGEA5-Antibody-Immunoprecipitation-NBP2-32233-img0003.jpg "Immunoprecipitation: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233]")
Immunoprecipitation: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233]
Immunoprecipitation: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MGEA5 antibody NBP2-32233 used for IP at 6 ug per reaction. MGEA5 was also immunoprecipitated by rabbit anti-MGEA5 antibody BL15772. For blotting immunoprecipitated MGEA5, NBP2-32233 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot- O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] -
Western Blot- O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Human MGEA5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MGEA5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.Applications for O-GlcNAcase/OGA/MGEA5 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:5000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: O-GlcNAcase/OGA
Alternate Names
GH84, HEXC3, Hexosaminidase B, MGEA5, NCOAT, OGA, OGlcNAcase
Gene Symbol
OGA
UniProt
Additional O-GlcNAcase/OGA Products
Product Documents for O-GlcNAcase/OGA/MGEA5 Antibody - BSA Free
Product Specific Notices for O-GlcNAcase/OGA/MGEA5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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