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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Western Blot

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

E. coli-derived recombinant rat Nucleostemin
Lys2-Ile538
Accession # Q811S9

Specificity

Detects human, mouse, rat Nucleostemin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat Nucleostemin C-terminal peptide (aa 281-538) is observed. Rat Nucleostemin specific IgG was purified by first passing the sera over a rat Nucleostemin aa 2-538 column and then passing the bound fraction over a rat Nucleostemin aa 281-538 column to removed rat Nucleostemin C-terminal specific IgG. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Nucleostemin Antibody [mFluor Violet 450 SE]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Nucleostemin

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

Alternate Names

GNL3

Gene Symbol

GNL3

Additional Nucleostemin Products

Product Documents for Nucleostemin Antibody [mFluor Violet 450 SE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nucleostemin Antibody [mFluor Violet 450 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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