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Nucleoli Marker Antibody (NM95) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45215

Novus Biologicals, part of Bio-Techne
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NBP2-45215

Key Product Details

Species Reactivity

Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NM95

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Nuclei of myeloid leukemia biopsy cells

Reactivity Notes

Does not react with Mouse, Rat or Bovine.

Localization

Nucleoli

Specificity

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Marker

Marker For Human Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Nucleoli Marker Antibody (NM95) - IHC-Prediluted

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215]

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215]

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215] - Staining of human colon ca.
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215]

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215]

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nucleoli Marker Antibody (NM95).
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215]

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215]

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleoli Marker Antibody (NM95).

Applications for Nucleoli Marker Antibody (NM95) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.25 - 0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Nucleoli Marker

Additional Nucleoli Marker Products

Product Documents for Nucleoli Marker Antibody (NM95) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nucleoli Marker Antibody (NM95) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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