Nucleoli Marker Antibody (NM95)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32886
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # NM95
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Nuclei of myeloid leukemia biopsy cells
Reactivity Notes
Does not react with Mouse, Rat or Bovine.
Localization
Nucleoli
Specificity
This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Marker
Marker For Human Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34695)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Nucleoli Marker Antibody (NM95)
Immunocytochemistry/ Immunofluorescence: Nucleoli Marker Antibody (NM95) [NBP2-32886]
Immunocytochemistry/Immunofluorescence: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Immunofluorescence Analysis of PFA-fixed K562 cells labeling Nucleolin with followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886]
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleoli Marker Antibody (NM95).Flow Cytometry: Nucleoli Marker Antibody (NM95) [NBP2-32886]
Flow Cytometry: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleoli Marker Antibody (NM95)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)Applications for Nucleoli Marker Antibody (NM95)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Frozen
0.5-1.0ug/ml
Immunohistochemistry-Paraffin
0.25-0.5 ug/ml
Application Notes
Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT.
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Nucleoli Marker
Additional Nucleoli Marker Products
Product Documents for Nucleoli Marker Antibody (NM95)
Product Specific Notices for Nucleoli Marker Antibody (NM95)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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