Nuclear Antigen Antibody (rNM106)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13909
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (rNM106) [NBP3-13909]](https://resources.bio-techne.com/images/products/Nuclear-Antigen-Antibody-rNM106-Immunohistochemistry-Paraffin-NBP3-13909-img0001.jpg "Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (rNM106) [NBP3-13909]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rNM106
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Nuclei of HL60 cells
Reactivity Notes
Does not react with Porcine.
Localization
Nucleus
Specificity
This monoclonal antibody is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Marker
Pan-Nuclear Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14097)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Nuclear Antigen Antibody (rNM106)
Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (rNM106) [NBP3-13909]
Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (rNM106) [NBP3-13909] - Formalin-fixed, paraffin-embeddedhuman tonsil stained with Nuclear Antigen antibody (rNM106).Applications for Nuclear Antigen Antibody (rNM106)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Nuclear Antigen
Alternate Names
Nuclei, Nucleus
Additional Nuclear Antigen Products
Product Documents for Nuclear Antigen Antibody (rNM106)
Product Specific Notices for Nuclear Antigen Antibody (rNM106)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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