Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # NM2984R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Nuclear Antigen Antibodies »

Product Specifications

Immunogen

Nuclei of HL60 cells

Reactivity Notes

Does not react with Porcine.

Localization

Nuclei

Specificity

This monoclonal antibody is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Marker

Pan-Nuclear Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07303).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Nuclear Antigen Antibody (NM2984R) - Azide and BSA Free

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM2984R) - Azide and BSA Free [NBP3-08513]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM2984R) - Azide and BSA Free [NBP3-08513]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM2984R) - Azide and BSA Free [NBP3-08513] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nuclear Antigen Monoclonal Antibody (NM2984R).
Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM2984R) - Azide and BSA Free [NBP3-08513]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM2984R) - Azide and BSA Free [NBP3-08513]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM2984R) - Azide and BSA Free [NBP3-08513] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nuclear Antigen Monoclonal Antibody (NM2984R).

Applications for Nuclear Antigen Antibody (NM2984R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Nuclear Antigen

Alternate Names

Nuclei, Nucleus

Additional Nuclear Antigen Products

Product Documents for Nuclear Antigen Antibody (NM2984R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nuclear Antigen Antibody (NM2984R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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