Nuclear Antigen Antibody (NM106)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45273

Novus Biologicals, part of Bio-Techne
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NBP2-45273-0.02mg
NBP2-45273-0.1mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NM106

Concentration

0.2 mg/ml

View all Nuclear Antigen Antibodies »

Product Specifications

Immunogen

Nuclei of HL60 cells

Reactivity Notes

Does not react with Porcine.

Localization

Nuclei

Specificity

This monoclonal antibody is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Marker

Pan-Nuclear Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11613)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Nuclear Antigen Antibody (NM106)

Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (NM106) [NBP2-45273]

Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (NM106) [NBP2-45273]

Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (NM106) [NBP2-45273] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with Pan Nuclear Antigen Antibody followed by goat anti-Mouse IgG-CF488 (Green). Membranes are labeled with Phalloidin (Red).
Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273] - Analysis of mouse testis tissue using Nuclear Antigen antibody. Primary antibody dilution: 0.5 ug/ml dilution. Image from verified customer review.
Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) [NBP2-45273] - Human Tonsil stained with Pan Nuclear Antigen Antibody
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Applications for Nuclear Antigen Antibody (NM106)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-45273 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Nuclear Antigen

Alternate Names

Nuclei, Nucleus

Additional Nuclear Antigen Products

Product Documents for Nuclear Antigen Antibody (NM106)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nuclear Antigen Antibody (NM106)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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