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Key Product Details

Species Reactivity

Human, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 235-1R

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for Nuclear Antigen Antibody (235-1R) - Azide and BSA Free

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Reactivity Notes

Does not react with mouse, rat and chicken.

Localization

Nuclear

Specificity

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Marker

Human Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

70,80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13912).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for Nuclear Antigen Antibody (235-1R) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (235-1R) - Azide and BSA Free [NBP3-14102] - Immunofluorescence Analysis of MeOH-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green).
Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (235-1R) - Azide and BSA Free [NBP3-14102] - Immunofluorescence Analysis of PFA-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-mouse IgG-CF488 (green).

Applications for Nuclear Antigen Antibody (235-1R) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Frozen

1-2 ug/ml
Application Notes
Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT

Immunohistology (Frozen): 1-2ug/ml for 30 minutes at RT

Immunoprecipitation: 1-2 g/500ug protein lysate)
Immunoprecipitation: 1-2 gram/500 ug protein lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Nuclear Antigen

Alternate Names

Nuclei, Nucleus

Product Documents for Nuclear Antigen Antibody (235-1R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nuclear Antigen Antibody (235-1R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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