Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all REST/NRSF Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 800 and 850 of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NRSF Antibody - BSA Free

Immunohistochemistry-Paraffin: NRSF Antibody [NB100-756]

Immunohistochemistry-Paraffin: NRSF Antibody [NB100-756]

Immunohistochemistry-Paraffin: NRSF Antibody [NB100-756] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REST used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: NRSF Antibody [NB100-756]

Immunoprecipitation: NRSF Antibody [NB100-756]

Immunoprecipitation: NRSF Antibody [NB100-756] - Detection of human REST by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REST antibody NB100-756 used for IP at 6 ug per reaction. For blotting immunoprecipitated REST, NB100-756 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for NRSF Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-10 ug/mg lysate
Application Notes
Western Blot Not recommended. Use rabbit anti-REST antibody NB100-757. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: REST/NRSF

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.

Long Name

RE1-Silencing Transcription Factor

Alternate Names

NRSF, XBR

Entrez Gene IDs

5978 (Human)

Gene Symbol

REST

UniProt

Q13127 (Human)

Additional REST/NRSF Products

Product Documents for NRSF Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NRSF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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